3CB5

Crystal Structure of the S. pombe Peptidase Homology Domain of FACT complex subunit Spt16 (form A)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM16
Synchrotron site	ESRF
Beamline	BM16
Temperature [K]	100
Detector technology	CCD
Collection date	2007-02-28
Detector	ADSC QUANTUM 210
Wavelength(s)	0.931
Spacegroup name	P 21 21 21
Unit cell lengths	97.070, 101.650, 103.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.120 - 2.050
R-factor	0.20767
Rwork	0.206
R-free	0.24466
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	1.419
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SOLVE
Refinement software	REFMAC (5.4.0067)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.550	2.170
High resolution limit [Å]	2.050	2.050
Number of reflections	64731
<I/σ(I)>	11	2.19
Completeness [%]	99.6	99
Redundancy	3.65	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	13% PEG 2000, 0.1M Bis-Tris propane, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K
1	VAPOR DIFFUSION, HANGING DROP	7	293	13% PEG 2000, 0.1M Bis-Tris propane, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K
1	VAPOR DIFFUSION, HANGING DROP	7	293	13% PEG 2000, 0.1M Bis-Tris propane, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K