3CAM

Crystal structure of the cold shock domain protein from Neisseria meningitidis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2006-07-19
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97900
Spacegroup name	P 41 21 2
Unit cell lengths	59.369, 59.369, 89.423
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.600
R-factor	0.23646
Rwork	0.233
R-free	0.29663
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.022
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHELXCD
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.127	0.834
Number of reflections	5271
<I/σ(I)>	29.2	1.7
Completeness [%]	98.5	88.8
Redundancy	23.9	13.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.7	293	0.8 M Sodium dihydrogen phosphate, 1.2 M di-potassium hydrogen phosphate, 100 mM Acetate pH 4.5, pH 6.7, VAPOR DIFFUSION, SITTING DROP, temperature 293K