3CAF
Crystal Structure of hFGFR2 D2 Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2006-10-27 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 42.500, 79.900, 37.290 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 16.760 - 1.960 |
| R-factor | 0.191 |
| Rwork | 0.187 |
| R-free | 0.27500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.032 |
| RMSD bond angle | 2.082 |
| Data reduction software | CrystalClear |
| Data scaling software | d*TREK (9.2SSI) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 16.760 | 16.760 | 2.030 |
| High resolution limit [Å] | 1.960 | 4.200 | 1.960 |
| Rmerge | 0.048 | 0.021 | 0.386 |
| Total number of observations | 3587 | 1747 | |
| Number of reflections | 9401 | ||
| <I/σ(I)> | 14.4 | 51.7 | 1.9 |
| Completeness [%] | 98.1 | 99.7 | 84.9 |
| Redundancy | 3.39 | 3.39 | 2.22 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 20%(w/v) PEG3350, 0.1M (NH3)2SO4, 100mM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
