3CAD
Crystal structure of Natural Killer Cell Receptor, Ly49G
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-02-28 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 133.378, 56.196, 33.761 |
Unit cell angles | 90.00, 100.29, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.600 |
R-factor | 0.22971 |
Rwork | 0.227 |
R-free | 0.28636 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.018 |
RMSD bond angle | 1.777 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.069 | 0.100 |
Number of reflections | 8105 | |
<I/σ(I)> | 7.3 | |
Completeness [%] | 81.5 | 45 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 30% (weight/volume) PEG 4000, 0.1 mM Tris-HCl (pH 8.5), and 0.2 M MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |