3C90
The 1.25 A Resolution Structure of Phosphoribosyl-ATP Pyrophosphohydrolase from Mycobacterium tuberculosis, crystal form II
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | MACSCIENCE |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | MAC Science DIP-2030 |
Wavelength(s) | 1.54178 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 117.702, 43.119, 67.547 |
Unit cell angles | 90.00, 96.43, 90.00 |
Refinement procedure
Resolution | 33.560 - 1.790 |
R-factor | 0.209 |
Rwork | 0.207 |
R-free | 0.25600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1y6x |
RMSD bond length | 0.010 |
RMSD bond angle | 1.152 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.790 |
Number of reflections | 31912 |
Completeness [%] | 98.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1M Sodium formate, 0.1M Sodium iodide, 0.1 mM Calcium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 293K |