3C8C
Crystal structure of Mcp_N and cache domains of methyl-accepting chemotaxis protein from Vibrio cholerae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2008-02-08 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 103.202, 140.942, 32.221 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.500 |
| R-factor | 0.19495 |
| Rwork | 0.194 |
| R-free | 0.23452 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.285 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.3.0034) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.140 | 1.540 |
| High resolution limit [Å] | 1.460 | 1.460 |
| Rmerge | 0.108 | 0.920 |
| Number of reflections | 81575 | |
| <I/σ(I)> | 13.1 | 2.4 |
| Completeness [%] | 96.1 | 95.6 |
| Redundancy | 13.7 | 11.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 100mM Sodium cacodylate pH 6.5, 200mM Sodium acetate, 30% PEG 8000, 10% Glycerol, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 294K |
