3C7Y
Mutant R96A OF T4 lysozyme in wildtype background at 298K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1998-05-07 |
| Detector | SDMS |
| Wavelength(s) | 1.5414 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 61.039, 61.039, 97.092 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.000 - 1.950 |
| R-factor | 0.211 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l63 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.410 |
| Data reduction software | san (Deigo Multiwire) |
| Data scaling software | San (Deigo Multiwire) |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.000 | 1.980 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.080 | |
| Number of reflections | 14074 | |
| Completeness [%] | 89.0 | 83.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 277 | 2 M NA/K PHOSPHATE PH 6.7 550 mM NACL 50 MM REDUCED BME, 50 MM OXIDIZED BME , VAPOR DIFFUSION, HANGING DROP, temperature 277K |
