3C7L
Molecular architecture of Galphao and the structural basis for RGS16-mediated deactivation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-10-01 |
Detector | BRANDEIS - B4 |
Wavelength(s) | 1.10 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.379, 71.700, 72.306 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.890 |
Rwork | 0.222 |
R-free | 0.26800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1agr |
RMSD bond length | 0.005 |
RMSD bond angle | 1.021 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.890 |
Number of reflections | 20395 |
<I/σ(I)> | 21.3 |
Completeness [%] | 94.6 |
Redundancy | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 34% PEG 400 150 mM ammonium citrate 100 mM cacodylate pH 6.5 0.5 mM KCl6Ir(IV), VAPOR DIFFUSION, HANGING DROP, temperature 277K |