3C74
X-ray structure of the uridine phosphorylase from salmonella typhimurium in complex with 2,2'-anhydrouridine at 2.38a resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X13 |
Detector technology | CCD |
Collection date | 2006-11-01 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.0500 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 90.370, 125.308, 135.177 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.940 - 2.380 |
R-factor | 0.20944 |
Rwork | 0.208 |
R-free | 0.25787 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.154 |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 2.380 |
Number of reflections | 61088 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 297 | PEG400, NaN3, VAPOR DIFFUSION, HANGING DROP, temperature 297K |