3C6N

Small molecule agonists and antagonists of F-box protein-substrate interactions in auxin perception and signaling

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-07-21
Detector	ADSC QUANTUM 315
Spacegroup name	C 1 2 1
Unit cell lengths	102.510, 80.661, 125.071
Unit cell angles	90.00, 104.95, 90.00

Refinement procedure

Resolution	49.520 - 2.600
R-factor	0.19
Rwork	0.185
R-free	0.28100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1p1m
RMSD bond length	0.035
RMSD bond angle	3.422
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.600	2.600
Number of reflections	26541
<I/σ(I)>	22.8
Completeness [%]	75.0	75
Redundancy	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6	277	12-14% PEG 40,000 1mM BTP pH 6.0, 200mM NaCl, EVAPORATION, temperature 277K, pH 6.00