3C6H
Crystal Structure of the RB49 gp17 nuclease domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-10-01 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.033 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 96.436, 126.724, 52.391 |
Unit cell angles | 90.00, 106.03, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.800 |
R-factor | 0.242 |
Rwork | 0.242 |
R-free | 0.29000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3c6a |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.041 | 0.139 |
Number of reflections | 29056 | |
<I/σ(I)> | 18.6 | 8.7 |
Completeness [%] | 95.0 | 86.9 |
Redundancy | 3.1 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 15% PEG400, 0.2M KCl, 50mM Tris pH 8.5, 10mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |