3C6F
Crystal structure of protein Bsu07140 from Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2006-10-11 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.268, 71.334, 70.094 |
| Unit cell angles | 90.00, 111.93, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.2346 |
| Rwork | 0.233 |
| R-free | 0.28763 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.108 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.3.0034) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Rmerge | 0.060 | 0.770 |
| Number of reflections | 31443 | |
| <I/σ(I)> | 5.7 | 0.4 |
| Completeness [%] | 96.1 | 89.2 |
| Redundancy | 1.8 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294 | 50mM Bis-Tris pH 6.5, 25% Pentaerythriol ethoxylate, 100 mM Ammonium sulfate, 10% Glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
