3C5Q

Crystal structure of diaminopimelate decarboxylase (I148L mutant) from Helicobacter pylori complexed with L-lysine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2007-12-29
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 32 2 1
Unit cell lengths	79.111, 79.111, 134.821
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	14.980 - 2.400
Rwork	0.202
R-free	0.25300
RMSD bond length	0.007
RMSD bond angle	1.304
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.140	0.373
Total number of observations		14447
Number of reflections	19223
<I/σ(I)>	4.9	1.9
Completeness [%]	98.2	99.9
Redundancy	7.33	7.45

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	0.1M Bicine, 1.6M Ammonium Sulfate, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K