3C57
Crystal Structure of the Mycobacterium tuberculosis Hypoxic Response Regulator DosR C-terminal Domain Crystal Form II
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.637, 47.400, 66.436 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.580 - 1.700 |
R-factor | 0.1894 |
Rwork | 0.189 |
R-free | 0.20612 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | DosR C-terminal domain (pdb code 1ZLJ) subunit A residues 152-192 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.330 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 14128 | |
<I/σ(I)> | 19.3 | 2.2 |
Completeness [%] | 98.5 | |
Redundancy | 6.43 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 24% w/v PEG 5000mme, 0.2M ammonium sulfate, 10% glycerol, 0.1M MES pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |