3C4Y
Crystal Structure of Apo form of G protein coupled receptor kinase 1 at 7.51A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97967 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 123.313, 123.313, 192.517 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 7.509 |
| R-factor | 0.18216 |
| Rwork | 0.182 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3c4w |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.999 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 7.500 |
| Number of reflections | 2383 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 277 | PEG 3350, NaCl, MES pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
