3C4T
Structure of RNaseIIIb and dsRNA binding domains of mouse Dicer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-05 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.11 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 70.126, 70.126, 132.663 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.800 |
| R-factor | 0.23568 |
| Rwork | 0.233 |
| R-free | 0.30429 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.163 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0067) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.850 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.057 | 0.368 |
| Number of reflections | 7819 | |
| <I/σ(I)> | 28.6 | 3.67 |
| Completeness [%] | 86.0 | 46 |
| Redundancy | 7.5 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 20-26% PEG400, 100 mM HEPES (pH7.0), VAPOR DIFFUSION, HANGING DROP, temperature 295K |
