3C46
X-ray crystal structure of the N4 mini-vRNAP P2_7a promoter complex soaked with MgCl2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-30 |
| Detector | ADSC Q315 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 82.622, 111.424, 276.763 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| Rwork | 0.210 |
| R-free | 0.24200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.081 | 0.045 | 0.472 |
| Number of reflections | 148149 | ||
| <I/σ(I)> | 12 | ||
| Completeness [%] | 85.5 | 95.3 | 75.9 |
| Redundancy | 3 | 3.2 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 294 | 0.1 M Tris-HCl (pH 8.0), 0.21 M dibasic ammonium citrate, 0.09 M ammonium dihydrogen monophosphate, 25 % (drop) or 15% (reservoir) PEG 3350, hanging drop, temperature 294K, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Tris-HCl | ||
| 2 | 1 | 1 | dibasic ammonium citrate | ||
| 3 | 1 | 1 | ammonium dihydrogen monophosphate | ||
| 4 | 1 | 1 | PEG 3350 | ||
| 5 | 1 | 2 | Tris-HCl | ||
| 6 | 1 | 2 | dibasic ammonium citrate | ||
| 7 | 1 | 2 | ammonium dihydrogen monophosphate | ||
| 8 | 1 | 2 | PEG 3350 |
