3C44
Crystal structure of HIV-1 subtype F DIS extended duplex RNA bound to paromomycin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2004-07-22 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9195 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 30.818, 95.703, 47.856 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.550 - 2.000 |
| R-factor | 0.224 |
| Rwork | 0.224 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3c3z |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 9333 | |
| <I/σ(I)> | 25.7 | 6.2 |
| Completeness [%] | 92.0 | 77.1 |
| Redundancy | 3.7 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 310 | MPD, MgCl2, KCl, Na cacodylate, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 310K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Na cacodylate | ||
| 2 | 1 | 1 | MgCl2 | ||
| 3 | 1 | 1 | KCl | ||
| 4 | 1 | 1 | MPD | ||
| 5 | 1 | 2 | Na cacodylate | ||
| 6 | 1 | 2 | MgCl2 | ||
| 7 | 1 | 2 | KCl | ||
| 8 | 1 | 2 | MPD |
