3C3K
Crystal structure of an uncharacterized protein from Actinobacillus succinogenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97900 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.888, 67.602, 204.802 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.470 - 1.990 |
| R-factor | 0.183 |
| Rwork | 0.180 |
| R-free | 0.23100 |
| Structure solution method | SAD |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.994 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | RESOLVE (2.13) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.060 |
| High resolution limit [Å] | 1.990 | 4.290 | 1.990 |
| Rmerge | 0.088 | 0.048 | 0.245 |
| Number of reflections | 55962 | ||
| <I/σ(I)> | 8.4 | ||
| Completeness [%] | 86.4 | 99.7 | 38.4 |
| Redundancy | 3 | 3.6 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 294 | 0.2 M Magnesium chloride, 0.1 M Bis-tris, 25% PEG 3350, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
