3C3D

Crystal structure of 2-phospho-(S)-lactate transferase from Methanosarcina mazei in complex with Fo and phosphate. Northeast Structural Genomics Consortium target MaR46

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-11-09
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97916
Spacegroup name	P 32
Unit cell lengths	185.278, 185.278, 67.789
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.590 - 2.500
R-factor	0.195
Rwork	0.195
R-free	0.23100
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SnB
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.590	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.088	0.410
Number of reflections	178601
<I/σ(I)>	15.37	4.15
Completeness [%]	99.1	98
Redundancy	5.1	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	Protein solution: 10 mM Tris-HCl pH 7.5, 100 mM NaCl, 5 mM Fo, 5 mM Lactyl phosphate, 5 mM DTT. Reservoir solution: 4.2 M Sodium formate, VAPOR DIFFUSION, HANGING DROP, temperature 291K