3C34

Crystal structure of GluR5 ligand-binding core in complex with rubidium at 1.82 Angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2007-11-14
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00000
Spacegroup name	P 41 21 2
Unit cell lengths	70.952, 70.952, 234.326
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.100 - 1.820
R-factor	0.16848
Rwork	0.166
R-free	0.20897
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	3c31
RMSD bond length	0.019
RMSD bond angle	1.653
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	REFMAC (5.4.0066)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.890
High resolution limit [Å]	1.820	1.820
Rmerge	0.075	0.423
Number of reflections	54555
<I/σ(I)>	13.9	5.22
Completeness [%]	99.5	100
Redundancy	6.3	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	20% PEG 4000, 125 mM RbCl, 175 mM Rb2(SO4), 100 mM Rb cacodylate, 4 mM Kainic acid, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K