3C30

Crystal structure of the Vibrio Cholerae LuxQ periplasmic domain (SeMet)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	298
Detector technology	CCD
Collection date	2003-02-19
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97914
Spacegroup name	P 41 21 2
Unit cell lengths	55.584, 55.584, 160.959
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.860 - 2.800
R-factor	0.201
Rwork	0.201
R-free	0.22800
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	27.860
High resolution limit [Å]	2.800
Number of reflections	11874
<I/σ(I)>	21.8
Completeness [%]	99.8
Redundancy	12

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	12% v/v PEG 20000, 0.1M MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K