3C22
Crystal structure of the carbohydrate recognition domain of human Langerin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97966 |
| Spacegroup name | P 42 |
| Unit cell lengths | 79.555, 79.555, 90.144 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.720 - 1.500 |
| R-factor | 0.192 |
| Rwork | 0.189 |
| R-free | 0.23600 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.516 |
| Data reduction software | XDS |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.590 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.044 | 0.331 |
| Number of reflections | 89721 | |
| <I/σ(I)> | 25.4 | 6.1 |
| Completeness [%] | 99.4 | 98.7 |
| Redundancy | 7.2 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 400mM MgCl2, 25% PEG 3350, 100mM HEPES pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
