3C21
Structure of a bacterial DNA damage sensor protein with reaction product
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-06 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0099 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 109.080, 109.080, 165.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.700 |
| Rwork | 0.208 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3c1y |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.120 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.860 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.075 | 0.355 |
| Number of reflections | 27866 | |
| <I/σ(I)> | 24.51 | 7.19 |
| Completeness [%] | 98.2 | 96.6 |
| Redundancy | 9.63 | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1M Tris, 0.2M Ammoniumacetate, 35% MPD, 3mM ATP-gammaS, 3mM MgCl2, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
