3C1C
The effect of H3 K79 dimethylation and H4 K20 trimethylation on nucleosome and chromatin structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 292 |
| Detector technology | CCD |
| Collection date | 2006-09-07 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 107.030, 110.080, 182.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.000 - 3.150 |
| Rwork | 0.220 |
| R-free | 0.29000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aoi |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.382 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.000 | 3.260 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.088 | 0.285 |
| Number of reflections | 34295 | |
| <I/σ(I)> | 10.7 | 3.8 |
| Completeness [%] | 90.0 | 90 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 292 | Manganese chloride, Potassium chloride, Potassium cacodylate, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Manganese chloride | ||
| 2 | 1 | 1 | Potassium cacodylate | ||
| 3 | 1 | 2 | Potassium cacodylate | ||
| 4 | 1 | 2 | Potassium chloride |
