3C15
Complex of GS-Alpha with the Catalytic Domains of Mammalian Adenylyl Cyclase: Complex with Pyrophosphate and Mg
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0393 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 117.510, 133.080, 70.108 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.490 - 2.780 |
| R-factor | 0.23981 |
| Rwork | 0.237 |
| R-free | 0.29145 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1azs |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.128 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.900 |
| High resolution limit [Å] | 2.780 | 2.780 |
| Number of reflections | 25942 | |
| <I/σ(I)> | 1.8 | |
| Redundancy | 3.1 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 289 | 7.5-7.8% PEG 8000, 0.5M NACL, 0.1M PHOSPHATE BUFFER, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
