3C12

Crystal Structure of FlgD from Xanthomonas campestris: Insights into the Hook Capping Essential for Flagellar Assembly

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL12B2
Synchrotron site	SPring-8
Beamline	BL12B2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-09-24
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97945
Spacegroup name	P 61 2 2
Unit cell lengths	116.710, 116.710, 71.820
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.270 - 2.510
R-factor	0.257
Rwork	0.257
R-free	0.27200
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.640
High resolution limit [Å]	2.500	2.540
Rmerge	0.056	0.480
Number of reflections	11527
<I/σ(I)>	33	3.3
Completeness [%]	99.0	100
Redundancy	11	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		295	VAPOR DIFFUSION, temperature 295K