3C0Y
Crystal structure of catalytic domain of human histone deacetylase HDAC7
Replaces: 2NVRReplaces: 2I4YExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 32 |
| Unit cell lengths | 81.750, 81.750, 148.895 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.440 - 2.100 |
| R-factor | 0.20499 |
| Rwork | 0.202 |
| R-free | 0.26404 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2NVR |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.787 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.056 | 0.419 |
| Number of reflections | 64241 | |
| <I/σ(I)> | 33 | 2.6 |
| Completeness [%] | 98.1 | 85.3 |
| Redundancy | 5.4 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 300 | 13 % PEG 3350, 0.1 M HEPES pH 7.5, 10 % Isopropanol, 1 mM DTT, VAPOR DIFFUSION, SITTING DROP, temperature 300K |
