3C0T

Structure of the Schizosaccharomyces pombe Mediator subcomplex Med8C/18

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2006-10-01
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.979
Spacegroup name	P 32 2 1
Unit cell lengths	111.016, 111.016, 68.640
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.400
Rwork	0.211
R-free	0.23140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	The starting model is the structure of Med18 alone which has not been deposited.
RMSD bond length	0.006
RMSD bond angle	1.293
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.530
High resolution limit [Å]	2.400	2.400
Number of reflections	19282
<I/σ(I)>	21.4	4.8
Completeness [%]	99.4	99.4
Redundancy	11	11.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	100mM Tris pH 8.5, 2M sodium formate, VAPOR DIFFUSION, HANGING DROP, temperature 293K