3C0K
Crystal Structure of a ribosomal RNA methyltranferase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791, 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.312, 80.875, 66.155 |
| Unit cell angles | 90.00, 98.91, 90.00 |
Refinement procedure
| Resolution | 42.250 - 2.000 |
| Rwork | 0.194 |
| R-free | 0.24200 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.243 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SnB |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.040 |
| Rmerge | 0.153 | |
| Number of reflections | 42783 | |
| <I/σ(I)> | 18.6 | |
| Completeness [%] | 75.0 | 90 |
| Redundancy | 3.6 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.8 | 298 | 30% (w/v) PEG 4000, 0.1M Tris-HCl pH 8.5, 0.2M sodium acetate, pH 8.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
