3C00

Crystal structural of the mutated G247T EscU/SpaS C-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-01-01
Detector	ADSC QUANTUM 315
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	26.969, 53.884, 59.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 1.410
R-factor	0.217
Rwork	0.216
R-free	0.23600
RMSD bond length	0.013
RMSD bond angle	1.503
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.470
High resolution limit [Å]	1.410	3.060	1.410
Rmerge	0.057	0.038	0.695
Number of reflections	17142
<I/σ(I)>	11.4
Completeness [%]	99.4	94.4	99.9
Redundancy	12.9	12.3	8.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	7.5	293	Tris, HEPES, Peg3350, NaCl, pH 7.5, Microbatch, temperature 293K