3BZV

Crystal structural of the mutated T264A EscU C-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-01-01
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0000
Spacegroup name	P 61 2 2
Unit cell lengths	52.086, 52.086, 155.184
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.130 - 1.940
R-factor	0.217
Rwork	0.215
R-free	0.26800
RMSD bond length	0.023
RMSD bond angle	2.108
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.010
High resolution limit [Å]	1.940	4.180	1.940
Rmerge	0.066	0.051	0.725
Number of reflections	9941
<I/σ(I)>	10.5
Completeness [%]	99.6	99.2	97.4
Redundancy	13	11	11.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	5.5	293	Li2SO4, Bis-Tris, Peg3350, NaCl, pH 5.5, Microbatch, temperature 293K