3BZ6
Crystal structure of a conserved protein of unknown function from Pseudomonas syringae pv. tomato str. DC3000
Replaces: 2NR3Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97929, 0.97943 |
| Spacegroup name | P 65 |
| Unit cell lengths | 72.025, 72.025, 73.119 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.540 - 2.210 |
| R-factor | 0.19788 |
| Rwork | 0.196 |
| R-free | 0.24085 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.630 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.540 | 2.250 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.091 | 0.391 |
| Number of reflections | 10229 | |
| <I/σ(I)> | 46 | 2.59 |
| Completeness [%] | 93.1 | 58.5 |
| Redundancy | 13.2 | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1M Sodium acetate, 20% Isopropanol, 20% PEG 4000, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
