3BZ5
Functional domain of InlJ from Listeria monocytogenes includes a cysteine ladder
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-27 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.817 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 159.272, 159.272, 159.272 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.280 - 2.700 |
| R-factor | 0.18512 |
| Rwork | 0.182 |
| R-free | 0.25261 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1o6v |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.815 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.280 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.112 | 0.391 |
| Number of reflections | 18486 | |
| <I/σ(I)> | 17.9 | 5.3 |
| Completeness [%] | 99.9 | |
| Redundancy | 11.2 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 298 | 2M Ammonium sulfate, 0.1M Na acetate, pH 4.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
