3BYR
Mode of Action of a Putative Zinc Transporter CzrB (Zn form)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-02 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.2827, 1.2818, 1.2567 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 50.750, 74.560, 43.940 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.430 - 1.800 |
| R-factor | 0.209 |
| Rwork | 0.207 |
| R-free | 0.25000 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.109 |
| Data reduction software | DPS |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.3.0021) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 7849 | |
| <I/σ(I)> | 20.1 | 4.6 |
| Completeness [%] | 98.2 | 96.4 |
| Redundancy | 7.3 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 277 | 28 mg/ml protein, 25 mM zinc chloride, 0.1 M Tris-Hcl, pH 7.50, BATCH, temperature 277K |
