3BYI
Crystal structure of human Rho GTPase activating protein 15 (ARHGAP15)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-04-16 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.95362 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 120.618, 130.142, 62.477 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.250 |
R-factor | 0.191 |
Rwork | 0.188 |
R-free | 0.24400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | assembly of PDB entries 1TX4 1ow3 1rgp 2ngr 1grn 1f7c 1am4 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.379 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.370 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.086 | 0.823 |
Number of reflections | 47518 | |
<I/σ(I)> | 15.4 | 0.9 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 7.1 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | 2.4M Na malate, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K |