3BY9
Crystal structure of the V. cholerae Histidine Kinase DctB Sensor Domain
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-19 |
| Wavelength(s) | 0.9637, 0.9788, 0.9791, 0.9946 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.311, 90.982, 114.196 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.780 - 1.700 |
| Rwork | 0.162 |
| R-free | 0.19900 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 3.440 | 1.600 |
| Rmerge | 0.057 | 0.032 | 0.395 |
| Number of reflections | 76821 | ||
| <I/σ(I)> | 11.5 | ||
| Completeness [%] | 98.4 | 98.6 | 91.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 277 | isopropanol, calcium acetate, pH 7.5, vapor diffusion, temperature 277K |
