3BY8
Crystal Structure of the E.coli DcuS Sensor Domain
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-04-07 |
| Wavelength(s) | 0.9678, 0.9787, 0.9793, 0.9918 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 86.111, 86.111, 35.207 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.190 - 1.450 |
| Rwork | 0.219 |
| R-free | 0.23200 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | DM (4.0) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 3.020 | 1.400 |
| Rmerge | 0.046 | 0.041 | 0.238 |
| Number of reflections | 29824 | ||
| <I/σ(I)> | 17 | ||
| Completeness [%] | 99.9 | 100 | 98.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.5 | 277 | PEG MME 2000, isopropanol, ammonium citrate, pH 4.5, vapor diffusion, temperature 277K |
