3BXZ
Crystal structure of the isolated DEAD motor domains from Escherichia coli SecA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97902 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 177.823, 71.361, 119.536 |
| Unit cell angles | 90.00, 128.82, 90.00 |
Refinement procedure
| Resolution | 46.575 - 3.000 |
| R-factor | 0.245 |
| Rwork | 0.245 |
| R-free | 0.30500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fsi |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.575 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.069 | 0.451 |
| Total number of observations | 12284 | |
| Number of reflections | 23500 | |
| <I/σ(I)> | 8.2 | 1.7 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 25% Ethylene glycol, 0.025M Spermidine, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
