3BXA

Monomeric Far-red Fluorescent Protein mKate Crystallized at pH 4.2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	I 4
Unit cell lengths	98.181, 98.181, 106.452
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.070 - 1.750
R-factor	0.182
Rwork	0.180
R-free	0.23300
RMSD bond length	0.015
RMSD bond angle	1.766
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.810
High resolution limit [Å]	1.750	3.770	1.750
Rmerge	0.041	0.020	0.522
Number of reflections	50567
<I/σ(I)>	13.4
Completeness [%]	99.6	99.9	99.1
Redundancy	7.7	7.7	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.2	293	17.5% w/v PEG 3350, 0.07M citric acid, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K