3BWU
Crystal structure of the ternary complex of FimD (N-Terminal Domain, FimDN) with FimC and the N-terminally truncated pilus subunit FimF (FimFt)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2006-04-28 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.88562 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 126.948, 126.948, 68.755 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.340 - 1.760 |
| Rwork | 0.175 |
| R-free | 0.21200 |
| Starting model (for MR) | 1ze3 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.635 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 33.000 | 33.000 | 1.820 |
| High resolution limit [Å] | 1.760 | 3.790 | 1.760 |
| Rmerge | 0.081 | 0.044 | 0.354 |
| Number of reflections | 63367 | ||
| <I/σ(I)> | 8.6 | ||
| Completeness [%] | 99.9 | 100 | 99.3 |
| Redundancy | 5.4 | 5.5 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 20 mM Tris/HCl pH 8.0, 20% PEG 8000, 50 mM succinic acid pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
