3BWR
SV40 VP1 pentamer in complex with GM1 oligosaccharide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-29 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 152.764, 93.087, 118.882 |
| Unit cell angles | 90.00, 105.60, 90.00 |
Refinement procedure
| Resolution | 29.760 - 2.250 |
| R-factor | 0.19206 |
| Rwork | 0.187 |
| R-free | 0.23318 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sva |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.814 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.4.0013) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.097 | 0.276 |
| Number of reflections | 68387 | |
| <I/σ(I)> | 11.2 | 1.9 |
| Completeness [%] | 89.8 | 53.8 |
| Redundancy | 8.7 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | RESERVOIR: 0.1 M TRIS, PH 8.5, 24% PEG3350. DROP: RESERVOIR MIXED 4:1 WITH 30% ETHYLENE GLYCOL. THIS WAS MIXED 1:1 WITH VP1 (11 MG/ML), VAPOR DIFFUSION, HANGING DROP, temperature 293K |
