3BWA
Crystal Structure of HLA B*3508 in complex with a HCMV 8-mer peptide from the pp65 protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-07 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.995, 81.738, 111.096 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.300 |
| R-factor | 0.18995 |
| Rwork | 0.189 |
| R-free | 0.20886 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bw9 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.132 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.035 | 0.231 |
| Number of reflections | 109900 | |
| <I/σ(I)> | 48.1 | 4.4 |
| Completeness [%] | 96.0 | 76.1 |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 277 | 0.2M ammonium acetate, 16% PEG 3350, pH 7.6, vapor diffusion, hanging drop, temperature 277K |
