3BW1
Crystal structure of homomeric yeast Lsm3 exhibiting novel octameric ring organisation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-25 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9793 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 83.743, 83.743, 141.931 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.040 - 2.500 |
| R-factor | 0.21694 |
| Rwork | 0.216 |
| R-free | 0.24296 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i81 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.125 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.250 | 2.570 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.086 | 1.405 |
| Number of reflections | 9073 | 1169 |
| <I/σ(I)> | 23.7 | 1.5 |
| Completeness [%] | 97.6 | 83.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.75M lithium sulfate, 8% (v/v) 2-methyl-2,4-pentanediol, 0.1M imidazole (pH 7.0), 10mM uridine-5-monophosphate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
