3BUZ
Crystal structure of ia-bTAD-actin complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-05 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.014, 126.344, 147.127 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.690 - 2.810 |
| R-factor | 0.22747 |
| Rwork | 0.224 |
| R-free | 0.29845 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1GIQ AND 1IJJ |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.198 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.690 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 26445 | |
| Completeness [%] | 99.4 | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 20% PEG1000, 0.1M MES(pH6.5), VAPOR DIFFUSION, HANGING DROP, temperature 277K |
