3BUO
Crystal structure of c-Cbl-TKB domain complexed with its binding motif in EGF receptor'
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-03-25 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 63.862, 110.173, 55.821 |
Unit cell angles | 90.00, 89.94, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.600 |
Rwork | 0.231 |
R-free | 0.27800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2cbl |
RMSD bond length | 0.009 |
RMSD bond angle | 1.326 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 2.690 |
High resolution limit [Å] | 2.600 | 5.600 | 2.600 |
Rmerge | 0.109 | 0.057 | 0.335 |
Number of reflections | 21945 | ||
<I/σ(I)> | 6.5 | ||
Completeness [%] | 91.9 | 98.4 | 83 |
Redundancy | 2.7 | 3.7 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.25M Na formate, 20% PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |