3BUI
Golgi mannosidase II D204A catalytic nucleophile mutant complex with Tris
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-05 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.912 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.259, 108.988, 137.569 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.250 |
| R-factor | 0.125 |
| Rwork | 0.121 |
| R-free | 0.15900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hty |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.032 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.280 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.079 | 0.316 |
| Number of reflections | 263244 | |
| <I/σ(I)> | 13.2 | 4.7 |
| Completeness [%] | 93.1 | 56.3 |
| Redundancy | 6.9 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Tris, NaCl, PEG6000, MPD; Tris is found in the active site under normal crystallization conditions, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
