3BT4
Crystal Structure Analysis of AmFPI-1, fungal protease inhibitor from Antheraea mylitta
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-02-01 |
| Detector | mar345 dtb |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 60.580, 60.580, 85.065 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.940 - 2.100 |
| R-factor | 0.25074 |
| Rwork | 0.249 |
| R-free | 0.29306 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.401 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.400 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 5816 | |
| <I/σ(I)> | 12.9 | 2.3 |
| Completeness [%] | 99.6 | 96.8 |
| Redundancy | 9.7 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | Ammonium sulfate, bis-Tris, PEG3350, pH6.5, vapor diffusion, hanging drop, temperature 298K, VAPOR DIFFUSION, HANGING DROP |
