3BSU

Hybrid-binding domain of human RNase H1 in complex with 12-mer RNA/DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2006-08-03
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97928
Spacegroup name	P 21 21 21
Unit cell lengths	45.492, 64.262, 140.322
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.100
Rwork	0.220
R-free	0.29000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.270
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.200
High resolution limit [Å]	2.100	2.100
Rmerge	0.061	0.396
Number of reflections	23892
<I/σ(I)>		3.9
Completeness [%]	96.3	95.6
Redundancy	3.6	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	294	1.2 M NaCl, 0.1 M HEPES (pH 7.5), VAPOR DIFFUSION, HANGING DROP, temperature 294K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	NaCl
2	1	1	Hepes
3	1	2	NaCl
4	1	2	Hepes