3BSG
Barley alpha-amylase isozyme 1 (AMY1) H395A mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-12-05 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9340 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 91.150, 72.230, 61.330 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.450 - 1.950 |
R-factor | 0.211 |
Rwork | 0.211 |
R-free | 0.26600 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1ht6 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.800 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.450 | 2.200 |
High resolution limit [Å] | 1.950 | 1.950 |
Number of reflections | 30228 | |
<I/σ(I)> | 13.2 | 5.06 |
Completeness [%] | 95.5 | 96 |
Redundancy | 4.1 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 290 | 0.1M MES buffer pH 6, 20%(w/v) PEG 8000, 3% methyl-pentane-diol, VAPOR DIFFUSION, HANGING DROP, temperature 290K |